Biochem Badass 101 page 15

Credits to Biochem students Carlos, Shenelle, Liniker, Latisha, et. al

This video started with a revision session of Glycolysis.

Things that we learnt before:

• Glycolysis is the splitting of Glucose (a 6 Carbon Sugar) into 2 Pyruvate molecules (2 3-Carbon molecules).
• It occurs in the cytosol of cells, he stated that Glycolysis takes place in the cytoplasm of the cell which is not true.
• It produces a net gain of 2 Atp and 2 NADH moleules.

In the video he told us that Glycolysis take place in the presence or absence of Oxygen (Aerobic or Anaerobic conditions).

New Material

• The Krebs Cycle is also known as the Citric Acid Cycle (substance found in orange juice).
• It takes place in the inner space of Mitochondria  (Power House of cells).
• Mitochondria has a double membrane outer and inner.
• The inner membrane of the mitochondria is called the ‘Crista’ (plural Cristae).
• Therefore it has 2 compartments

1. The outer compartment.
2. The Matrix.

• A combination of Acetyl CoA (2 Carbon molecule) and Oxaloacetic Acid( 4 Carbon molecule)  to form Citric Acid (6 Carbon molecule) is what enters the Krebs Cycle.
• All reactions are catalyzed by enzymes.
• Carbons are lost via Co2 gas.
• The Purpose of the Krebs Cycle is to generate ATP, NADH, FADH2 etc.
• NADH and FADH2 produced here will go into the ETC (Electron Transport Chain) to generate much more ATP than in the Glycolysis Pathway and the Krebs Cycle.

Net Gain:

• 14 Atps
• 10 NADHs
• 2 FADH2s
• 6 CO2

Below is a detailed picture of the Krebs Cycle.